Na-K pump current in the Amphiuma collecting tubule.
نویسندگان
چکیده
منابع مشابه
Na-K pump current in the Amphiuma collecting tubule
There is strong evidence supporting the hypothesis of an electrogenic Na-K pump in the basolateral membrane of several epithelia. Thermodynamic considerations and results in nonepithelial cells indicate that the current carried by the pump could be voltage dependent. In order to measure the pump current and to determine its voltage dependence in a tight epithelium, we have used the isolated per...
متن کاملIntracellular Na+ and K+ activities and membrane conductances in the collecting tubule of Amphiuma
Membrane potentials and conductances, and intracellular ionic activities were studied in isolated perfused collecting tubules of K+-adapted Amphiuma. Intracellular Na+ (aNai) and K+ (aKi) activities were measured, using liquid ion-exchanger double-barreled microelectrodes. Apical and basolateral membrane conductances were estimated by cable analysis. The effects of inhibition of the apical cond...
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Activities of Na channels and Na pumps were studied in the rat cortical collecting tubule (CCT) during manipulation of the animals' mineralocorticoid status in vivo using a low-Na diet, diuretics, or administration of exogenous aldosterone. Tubules were isolated and split open to expose the luminal membrane surface. Using the whole-cell patch-clamp technique, activities of the apical Na channel...
متن کاملRegulation of apical K and Na channels and Na/K pumps in rat cortical collecting tubule by dietary K
The patch-clamp technique was used to study the properties and the density of conducting K and Na channels in the apical membrane of rat cortical collecting tubule. The predominant K channel observed in cell-attached patches (SK channels) had an outward single-channel conductance (with LiCl in the pipette) of 10 pS. The inward conductance (with KCl in the pipette) was 42 pS. The channel had a h...
متن کاملBasolateral Na+ pump modulates apical Na+ and K+ conductances in rabbit cortical collecting ducts.
Previous studies indicated that an acute elevation of peritubular K+ enhances K+ secretion and Na+ reabsorption in the isolated perfused cortical collecting duct (CCD) from rabbit kidneys [S. Muto, G. Giebisch, and S. Sansom. Am. J. Physiol. 255 (Renal Fluid Electrolyte Physiol. 24): F108-F114, 1988]. To determine the underlying cellular mechanisms, we used microelectrode techniques to assess t...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1989
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.94.3.493